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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a crucial role in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been claimed but thorough nucleotide sequence data is not really still obtainable.
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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally has the epitope regarded through the antibody.
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This guidebook outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best results are...
Antibodies bound to the blocking peptide now not bind to the epitope around the goal protein. By comparing the staining in the blocked antibody versus the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Various cyclins show unique expression and degradation patterns which add to your temporal coordination of every mitotic event. Cyclin E forms a complex with and functions as being a regulatory subunit of CDK2, whose exercise is required for mobile cycle G1/S transition. This protein accumulates within the G1-S period boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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